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Biotransformation of 5NSA (a) and 5CSA (b) by cell extracts from E. coli Rosetta 2(pJS804). Reaction mixtures contained 1.3 mg/ml of protein. The concentrations (left) correspond to 5NSA or 5CSA and NO2− (a) or Cl− (b), while the peak area at 305 nm (right) corresponds to compounds I and II. mAU, milli-absorbance unit.
