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Purification of SucCDAm applying Q-Sepharose FF as revealed by SDS-PAGE (11.5% [wt/vol] acrylamide). Lane 1, crude extract of cells; lane 2, soluble protein fraction after 90 min of centrifugation at 100,000 × g and 4°C; lane M, low-molecular-weight calibration kit (GE Healthcare, Uppsala, Sweden); lane 3, purified SucCDAm eluted with 50 mM Tris-HCl (pH 7.4)–150 mM NaCl. The SDS-gel was stained with Coomassie brilliant blue R.
