Table 3.

Kinetic parameters of SucCD from A. mimigardefordensis strain DPN7^T and E. coli^a

Substrate	Mean ± SD
	Vmax (μmol min−1 mg−1)	Km (mM)
		A. mimigardefordensis	E. coli
Succinate	9.85 ± 0.14	0.143 ± 0.001	0.25
Itaconate	1.54 ± 0.15	0.448 ± 0.093	ND
3SP	0.12 ± 0.01	0.818 ± 0.046	ND
ATP	15.84 ± 0.12	0.083 ± 0.002	0.004
CoA	12.67 ± 0.40	0.045 ± 0.007	0.070

^aFor DPN7^T, activity was measured by quantifying ADP generation rates using pyruvate kinase and lactate dehydrogenase in a coupled enzyme assay. Kinetic measurements for the organic acids were performed in the presence of 1 mM ATP and 0.1 mM CoA. ATP and CoA measurements were carried out in the presence of 5 mM succinate, 1 mM ATP (for CoA kinetics), and 0.1 mM CoA (for ATP kinetics). V_max and K_m values were determined by obtaining sets of velocity versus the concentration data. A minimum of six concentrations was used in each set. All values are expressed as means ± standard deviations of triplicates. Data for E. coli are from Joyce et al. (20).