Table 2.

qRT-PCR analysis^a

No.	Gene name	SCO no.	Function of gene product	Factor of transcription in real-time PCR (mean ±SD)		DNA microarray (mean)b
				48 h	64 h	48 h	64 h
1	kasO	SCO6280	Regulatory protein	129.62 ± 25.51	6.85 ± 1.40	2.33	1.77
2	actII-ORF4	SCO5085	Actinorhodin cluster activator protein	2.23 ± 0.26	148.83 ± 70.12	5.65	13.65
3	ramS	SCO6682	Hypothetical protein	31.55 ± 8.06	725.77 ± 61.37	4.31	7.42
4	cdaR	SCO3217	Transcriptional regulator	12.70 ± 2.42	9.51 ± 1.00	2.31	2.22
5	redD	SCO5877	Transcriptional regulator	31.36 ± 6.49	15.33 ± 2.34	2.93	1.62
6	scbA	SCO6266	ScbA protein	13.07 ± 1.65	5.12 ± 1.58	2.21	3.74
7	bldN	SCO3323	RNA polymerase sigma factor	0.09 ± 0.01	0.06 ± 0.01	0.18	0.09
8	wblA	SCO3579	Regulatory protein	0.03 ± 0.01	0.07 ± 0.02	0.05	0.05
9	chpB	SCO7257	Secreted protein	0.05 ± 0.01	0.07 ± 0.01	0.05	0.07
10	ssgA	SCO3926	Regulator	0.06 ± 0.01	0.17 ± 0.02	0.29	0.27
11	bldD	SCO1489	DNA-binding protein	0.61 ± 0.05	0.46 ± 0.03	0.63	0.30
12	whiG	SCO5621	RNA polymerase sigma factor	0.32 ± 0.07	0.22 ± 0.05	0.19	0.22
13	cvnD9	SCO1627	ATP/GTP binding protein	0.27 ± 0.03	NDc	0.28	0.32
14	cvnD11	SCO0585	ATP/GTP binding protein	0.08 ± 0.06	ND	0.14	0.19

^aRNA samples isolated from cultures of M145 and the ohkA mutant grown on MS agar covered with cellophane were used in qRT-PCR. The experiments were done in triplicate and repeated three times with RNA from independent cultures. The values reported are the means (± standard deviations) of three independent qRT-PCR analyses.

^bAverages of two microarray signal ratios (mutant/wild type) from the specific 60-nt probe replicates for each gene.

^cND, not measured.