Fig. 1.

SDS-PAGE (12%) analysis of acid-soluble proteins from wild-type M. xanthus strain DK1622. Vegetative, liquid-grown cells (lanes 2 to 4) and 5-day-old fruiting-body spores (lanes 6 to 8) were washed in Tris-buffered saline, lysed by sonication, and subjected to precipitation by increasing concentrations of trichloracetic acid (TCA). Pelleted proteins were resuspended in running buffer and subjected to SDS-PAGE separation before Commassie blue staining. Protein pellets from 2.5% TCA (lanes 2 and 6), 5% TCA (lanes 3 and 7), and 25% TCA (lanes 4 and 8) are shown. Molecular mass standards are shown in lanes 1 and 5.