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Inactivation of sapA in M. xanthus. (A) Orientation of the sapA gene (mxan_7407) (dark arrow) in the M. xanthus chromosome relative to that of the surrounding genes (image from NCBI; http://www.ncbi.nlm.nih.gov). (B) Commassie blue-stained 12% SDS-PAGE gel showing 2.5% TCA-soluble proteins from 5-day-old fruiting bodies on TPM starvation agar. Lane 1, wild-type strain DK1622; lane 2, mutant strain JD0619. The positions of the SapA and SapB proteins are indicated by arrows.
