Fig. 6.

Glycerol-induced expression of MspB (mxan_2432) and MspC (mxan_6969) correlates with glycerol-induced spore formation. (A) Methylene blue-stained cells were photographed at ×1,000 using a phase-contrast microscope. Cells were exposed to 0.5 M glycerol for increasing lengths of time. (B) SDS-PAGE (12%) separation and Coomassie blue staining of 2.5% TCA-soluble proteins from DK1622 cells exposed to 0.5 M glycerol for 0 h (lane 1), 0.5 h (lane 2), 1 h (lane 3), 2 h (lane 4), 3 h (lane 5), and 4 h (lane 6). The positions of proteins MspB and MspC are indicated by arrows.