Fig. 1.

Growth curve analysis of PA14 and various transporter mutants on choline and glycine betaine. (A) Comparison of WT and ΔcbcV grown in MOPS-P medium amended with 20 mM either choline (solid lines) or glycine betaine (dashed lines). (B) Comparison of ΔbetT1, ΔbetT3, and ΔbetT1 ΔbetT3 grown in MOPS-P medium amended with 20 mM choline. (C) Comparison of ΔcbcV, ΔcbcV ΔbetT1, and ΔcbcV ΔbetT3 transporter mutants grown in MOPS-P medium amended with 20 mM choline. Values represent the mean optical densities at 600 nm from three independent replicates, and error bars depict the standard deviations. In some cases, the error bars are obscured by the symbol.