Fig. 5.

Contributions of BetT1 and BetT3 to cbcV expression in PA14. (A) qRT-PCR analysis of expression of cbcV in the wild type and ΔbetT1 ΔbetT3. The strains were grown in MOPS-P medium amended with 20 mM choline, and RNA was extracted from aliquots removed at the indicated time points. Expression levels were normalized to rpoD transcript levels and are presented as the fold induction compared to levels in the WT at 0 h (uninduced). Values are the mean fold induction of biological duplicates ± the standard deviation. (B) Comparison of PA14 and ΔbetT1 ΔbetT3 grown in MOPS-P medium amended with 20 mM choline. The cells were pregrown in medium with pyruvate (solid lines) or glycine betaine (dashed lines). Values are mean optical densities at 600 nm (n = 3).