Table 1.
Strains and plasmids
| Strain or plasmid | Description | Strain no. | Source or reference |
|---|---|---|---|
| P. aeruginosa PA14 strains | |||
| WT | P. aeruginosa PA14 wild type | DH122 | 25 |
| ΔcbcV | In-frame deletion mutant of cbcV | DH1578 | This study |
| ΔT1 (ΔbetT1) | In-frame deletion mutant of betT1 | DH1579 | This study |
| ΔT3 (ΔbetT3) | In-frame deletion mutant of betT3 | DH1580 | This study |
| ΔcbcVΔT1 | In-frame deletions of cbcV and betT1 | DH1584 | This study |
| ΔcbcVΔT3 | In-frame deletions of cbcV and betT3 | DH1581 | This study |
| ΔT1ΔT3 | In-frame deletions of betT1 and betT3 | DH1583 | This study |
| T1::pMQ | betT1::pMQ89 disruption mutant; Gmr | DH1593 | This study |
| ΔT3T1::pMQ | In-frame deletion mutant of betT3, betT1::pMQ89 disruption mutant; Gmr | DH1597 | This study |
| ΔcbcVΔT3T1::pMQ | ΔVΔT3 with a betT1::pMQ89 disruption mutation; Gmr | DH1596 | This study |
| ΔgbdR | In-frame deletion of gbdR | DH466 | 42 |
| betI::TnM | betTI::TnM; Gmr (mutant ID no. 42999) | DH1575 | 19 |
| Plasmids | |||
| pUCP22 | High-copy-number Pseudomonas-stabilized vector; Gmr | 32 | |
| pEX18Gm | Integrating vector in P. aeruginosa; Gmr | 31 | |
| pMQ89 | Suicide vector in P. aeruginosa; Gmr | 33 | |
| pME6041 | Broad-host-range vector; Kmr | 7 | |
| pcbcXWV | pME6041 with cbcXWV from P. aeruginosa PAO1; Kmr | 7 | |
| pbetT3 | pME6041 with betT3 from P. aeruginosa PAO1; Kmr | 6 | |
| pbetT1 | pUCP22 with PA14 betT1 ORF as defined by start site in original PAO1/PA14 annotations; Gmr | This study | |
| pbetT1N | pUCP22 with PA14 betT1 ORF as defined by alternative upstream start site; Gmr | This study |