Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| W3110 | λ− F− derived from E. coli K-12 | 11 |
| BW25113 | lacIqrrnBT14 ΔlacZWJ16hsdR514 | 4 |
| ΔaraBADAH33 ΔrhaBADLD78 | ||
| JW4135 | BW25113 purA::Kmr | Keio collectionb |
| MP402 | BW25113 purB::Kmr | 26 |
| JW2461 | BW25113 purC::Kmr | Keio collection |
| JW0511 | BW25113 purK::Kmr | Keio collection |
| JW2541 | BW25113 purL::Kmr | Keio collection |
| JW2491 | BW25113 guaA::Kmr | Keio collection |
| JW5401 | BW25113 guaB::Kmr | Keio collection |
| JW2651 | BW25113 nrdF::Kmr | Keio collection |
| JW4197 | BW25113 nrdD::Kmr | Keio collection |
| JW2788 | BW25113 recB::Kmr | Keio collection |
| TH1559 | K12 recBrecC sbcB | 23 |
| IH125 | W3110 adk(ts) | NIGb |
| SE1796 | W3110 purA::Kmr | This study, W3110 × P1(JW4135) |
| SE1888 | W3110 purB::Kmr | This study, W3110 × P1(MP402) |
| SE1797 | W3110 purC::Kmr | This study, W3110 × P1(JW2461) |
| SE1831 | W3110 purK::Kmr | This study, W3110 × P1(JW0511) |
| SE1832 | W3110 purL::Kmr | This study, W3110 × P1(JW2541) |
| SE1887 | W3110 guaA::Kmr | This study, W3110 × P1(JW2491) |
| SE1833 | W3110 guaB::Kmr | This study, W3110 × P1(JW5401) |
| SE1929 | W3110 recB::Kmr | This study, W3110 × P1(JW2788) |
| Plasmids | ||
| ppurA | pNTR-SD-purA | Mobile plasmid collectionb |
| ppurB | pNTR-SD-purB | Mobile plasmid collection |
a
Kmr, resistant to kanamycin.
b
Obtained from the National BioResource Project (NIG, Japan) for E. coli.