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. 2011 May;31(9):1833–1847. doi: 10.1128/MCB.01331-10

Fig. 10.

Fig. 10.

ChIP-seq analysis of KAP1 lacking both the N- and C-terminal domains. (A) ChIP-qPCR was performed using an antibody against the FLAG tag and HEK293 cells stably expressing FLAG-tagged Δ(RBBB+PB)-KAP1 (this KAP1 construct contains a deletion of both the N and the C termini of KAP1). Fold enrichment for Δ(RBBB+PB)-KAP1 (compared to 0.1% of the starting chromatin) is shown; results are from two independent replicates, and the error bars indicate standard errors. (B) Shown are ChIP-seq profiles for an example from each category of KAP1 binding sites for Δ(RBBB+PB)-KAP1. The number of sequenced tags is shown on the y axis, and the genomic coordinates are shown on the x axis. The arrows indicate the direction of transcription. (C) Snapshot of ChIP-seq data for FLAG-tagged Δ(RBBB+PB)-KAP1, compared to WT KAP1, at a region on chromosome 19. The number of sequenced tags is shown on the y axis, and the genomic coordinates are shown on the x axis. The arrows indicate the direction of transcription.