Fig. 3.

KAP1 binds to promoters and nonpromoter regions. (A) Five binding sites identified by ChIP-seq were selected from each of three different types of KAP1 targets, i.e., 3′ ends of ZNF genes, 5′ regions of ZNF genes, and 5′ regions of non-ZNF genes; 4 negative-control regions were also chosen. All regions were tested by ChIP-qPCR using an antibody against the FLAG peptide and HEK293 cells stably expressing FLAG-tagged WT KAP1. Fold enrichment for FLAG-tagged WT KAP1 (compared to 0.1% of the starting chromatin) is shown; results are from two independent replicates, and the error bars indicate standard errors. (B) Shown are ChIP-seq profiles for FLAG-tagged WT KAP1 for an example from each category of KAP1 binding sites. The number of sequenced tags is shown on the y axis, and the genomic coordinates are shown on the x axis. The arrow indicates the direction of transcription.