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. 2011 Jun;31(11):2196–2209. doi: 10.1128/MCB.01430-10

Fig. 2.

Fig. 2.

IL-4-mediated induction of VCAM-1 expression in the intact endothelium. Mice were pretreated with saline (control) or blocking antibody against IL-4Rα and then injected i.v. with control saline, 0.5 mg/kg IL-4, or 0.1 mg/kg TNF-α. Tissues were harvested 6 h later, and cryosections of the aorta (A and B) and heart (C) were collected and stained with anti-VCAM-1 (A and C) or anti-ICAM-1 (B) antibody (left panels, red), ICAM-2 (right panels, green), and DAPI (right panels, blue). Merged images are shown in the right panels. Bar, 50 μm. L, lumen. (D) Total RNA was harvested from hearts and assayed for VCAM-1 or ICAM-1 mRNA levels by quantitative real-time PCR. Ab, antibody. The results show the means and standard deviations of expression levels relative to cyclophilin A from at least four independent sets of mice. *, P < 0.001 compared with VCAM-1 or ICAM-1 expression levels with saline treatment. N.S., nonsignificant.