Table 2.
Dissociation constants for binding of full-length and H1 CTD deletion mutants to nucleosomes with linker DNAs differing by 1 bp in length
| DNA fragment size (bp) | Binding affinitya |
Linker DNA length (bp)b | |||||
|---|---|---|---|---|---|---|---|
| WT H1 (nM) | Δ22 (nM) | Δ44 (nM) | Δ66 (nM) | Δ88 (nM) | NG (nM) | ||
| 161 | >50 | >50 | >50 | >50 | >50 | >50 | 7 |
| 163 | >50 | >50 | >50 | 43.7 ± 7.0 | 4.6 ± 1.6 | 16.7 ± 2.9 | 8 |
| 165 | >50 | >50 | >50 | 3.4 ± 0.5 | 4.7 ± 1.4 | 19.3 ± 6.3 | 9 |
| 167 | 9.7 ± 1.3 | 8.4 ± 1.4 | 6.7 ± 1.2 | 4.2 ± 0.8 | 0.6 ± 0.1 | 8.3 ± 2.7 | 10 |
| 171 | 9.7 ± 1.0 | 7.4 ± 1.2 | 4.6 ± 0.6 | 4.3 ± 0.8 | 6.5 ± 0.8 | ND | 12 |
| 175 | 10.5 ± 3.0 | 6.3 ± 1.2 | 3.5 ± 0.8 | 2.4 ± 0.6 | 4.1 ± 1.0 | 12.5 ± 3.5 | 14 |
a
Binding affinities for full-length and H1 CTD deletion mutants. Specific binding constants of >50 nM were not determined due to nonspecific association. ND, not determined.
b
Length of each linker DNA, on each side of the 147-bp nucleosome core.