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. 2011 Jul;193(13):3186–3196. doi: 10.1128/JB.00272-11

Table 1.

Genes involved in sporulation controlled by SigH or differentially expressed at 4 h and 10 h of growth

Locus tag Gene namea Functiona Expression ratio in transcriptomeb
 Associated sigma factor(s) and/or Spo0A box(es)c
sigH strain/630E 4 h/10 h
CD0057 spo0H RNA polymerase sigma-H factor D 0.19 SigA*, Spo0A
CD1214 spo0A Stage 0 sporulation protein A 0.20 0.07 SigA*, SigH*, Spo0A
CD2492 Histidine kinase associated to Spo0A 0.35 0.55 SigH*
CD3670 Putative selenocysteine lyase 0.34 0.38
CD3671 spo0J Spo0J, stage 0 sporulation protein J 0.46 —
CD3672 soj Sporulation initiation inhibitor 0.56 — SigH
CD3673 Putative stage 0 sporulation protein, Spo0J-like 0.21 0.28 SigH
CD0770 spoIIAA Anti-sigma F factor antagonist 0.03 0.04 SigH*, Spo0A
CD0771 spoIIAB Anti-sigma F factor 0.03 0.04
CD0772 sigF RNA polymerase sigma-F factor 0.05 0.08
CD2469 spoIIP Stage II sporulation protein P 0.50 0.33
CD2470 gpr Spore endopeptidase 0.48 0.51
CD2641 Putative sporulation protein 0.48 0.59
CD2642 sigG RNA polymerase sigma-G factor 0.20 0.09 SigF*
CD2643 sigE RNA polymerase sigma-E factor 0.17 0.18
CD2644 spoIIGA Sporulation sigma-E factor processing peptidase 0.20 0.22
CD3490 spoIIE Phosphoprotein phosphatase 0.03 0.02 Spo0A
CD1192 spoIIIAA Stage III sporulation protein AA 0.31 0.42
CD1193 spoIIIAB Stage III sporulation protein AB 0.25 0.31
CD1194 spoIIIAC Stage III sporulation protein AC 0.16 0.18
CD1195 spoIIIAD Stage III sporulation protein AD 0.32 0.38
CD1196 spoIIIAE Stage III sporulation protein AE 0.35 0.41
CD1197 spiIIIAF Stage III sporulation protein AF 0.50 0.48
CD1198 spoIIIAG Stage III sporulation protein AG 0.14 0.15
CD1199 spoIIIAH Stage III sporulation protein AH 0.19 0.18
CD0126 spoIIID Stage III sporulation protein D 0.07 0.12
CD2629 spoIVA Stage IV sporulation protein AA 0.11 0.16
CD2656 spoVD Stage V sporulation protein D (penicillin-binding protein) 0.15 0.18
CD2652 spoVE Cell division/stage V sporulation protein 0.20 0.28
CD3516 spoVG Regulator required for spore cortex synthesis 0.01 0.23 SigH
CD1935 spoVS Stage V sporulation protein S 0.03 0.08 SigH
CD3499 spoVT Stage V sporulation protein T — 0.08
CD2967 spoVFB Dipicolinate synthase subunit B — 2.20
CD1230 sigK Fragment of RNA polymerase sigma-K factor (part 1) — 2.73
CD3563 Putative spore cortex-lytic enzyme 0.47 0.28
CD3494 Putative spore protein 0.50 0.42
CD0106 cwlD Germinaion N-acetylmuramoyl-l-alanine amidase 0.31 0.31
CD3349 bclA3 Putative exosporium glycoprotein 0.38 3.51 Spo0A
CD3569 Sporulation-specific protease 0.33 — SigH, Spo0A
CD1068 Putative polysaccharide biosynthesis/sporulation protein 0.39 0.4
CD3541 spmB Spore maturation protein B 0.43 —
CD2247 cspBA Serine peptidase 0.50 0.43
a

Gene names and functions correspond to those indicated in the MaGe database Clostriscope (https://www.genoscope.cns.fr).

b

A gene is considered differentially expressed when the P value is <0.05 using the statistical analysis described in Materials and Methods. The cutoff for biologically significant change was set at 2-fold. However, some genes had a fold change of less than 2-fold but were included because they appeared to be in the same transcription units with regulated genes for which the fold change was ≥2. Genes not differentially expressed between 4 h and 10 h of growth or between the 630E strain and the sigH mutant are indicated by a dash. D, deleted in the sigH mutant.

c

Using the B. subtilis SigH or Spo0A consensus, we searched for SigH-dependent promoters or Spo0A boxes. An asterisk indicates that we performed promoter cartography by 5′ RACE.