Table 4.
Gene | cDNA samplea | Mean CT ± SDc | Relative expressionb,c (%) |
---|---|---|---|
cbbM | UR2-auto | 16.2 ± 0.16 | 100 |
UR5311-auto | 25.2 ± 0.02 | 1.4 | |
UR2-hetero | 24.5 ± 0.06 | 0.8 | |
cbbE | UR2-auto | 18.7 ± 0.04 | 100 |
UR5311-auto | 22.0 ± 0.01 | 9.5 | |
UR2-hetero | 22.8 ± 0.03 | 2.3 | |
cbbP | UR2-auto | 21.4 ± 0.07 | 100 |
UR5311-auto | 25.5 ± 0.03 | 6.7 | |
UR2-hetero | 25.0 ± 0.12 | 3.7 |
cDNAs are from the R. rubrum wild type (UR2) and cbbR mutant (UR5311) grown under photoautotrophic growth conditions (auto) or photoheterotrophic conditions (hetero). The expressions of cbbM, cbbE, and cbbP in these two strains are compared.
Relative expression was calculated as described in Materials and Methods and then normalized by using the UR2-auto sample as a control for 100% expression.
The rank orders of samples for each of the genes in the “mean CT” (threshold cycle) and the “relative expression” analyses differ because the value for the reference gene, rpoD, varied slightly between heterotrophic and autotrophic growth conditions.