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. 2011 Jul;193(13):3293–3303. doi: 10.1128/JB.00265-11

Table 4.

Relative expression levels of cbb genes in the wild type (UR2) and the cbbR mutant (UR5311) based on qPCR data

Gene cDNA samplea Mean CT ± SDc Relative expressionb,c (%)
cbbM UR2-auto 16.2 ± 0.16 100
UR5311-auto 25.2 ± 0.02 1.4
UR2-hetero 24.5 ± 0.06 0.8
cbbE UR2-auto 18.7 ± 0.04 100
UR5311-auto 22.0 ± 0.01 9.5
UR2-hetero 22.8 ± 0.03 2.3
cbbP UR2-auto 21.4 ± 0.07 100
UR5311-auto 25.5 ± 0.03 6.7
UR2-hetero 25.0 ± 0.12 3.7
a

cDNAs are from the R. rubrum wild type (UR2) and cbbR mutant (UR5311) grown under photoautotrophic growth conditions (auto) or photoheterotrophic conditions (hetero). The expressions of cbbM, cbbE, and cbbP in these two strains are compared.

b

Relative expression was calculated as described in Materials and Methods and then normalized by using the UR2-auto sample as a control for 100% expression.

c

The rank orders of samples for each of the genes in the “mean CT” (threshold cycle) and the “relative expression” analyses differ because the value for the reference gene, rpoD, varied slightly between heterotrophic and autotrophic growth conditions.