Table 2.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pBluescriptSK(−) | E. coli cloning vector; Ampr | Stratagene |
| pGEX-6P-2 | E. coli GST tag expression vector; Ampr | Amersham |
| pET-42b(+) | E. coli His tag expression vector; Ampr | Novagen |
| pMUTIN-YFP | E. coli YFP fusion vector; Ampr Emr | 18 |
| pMUTIN-FLAG | E. coli FLAG fusion vector; Ampr Emr | 18 |
| pMUTIN-HIS | E. coli His tag fusion vector; Ampr Emr | This work |
| pHS101 | pGEX-6P-2::cheA1 | 41 |
| pHS102 | pGEX-6P-2::cheY1 | 41 |
| pUSH1 | B. subtilis-E. coli shuttle vector for His tag fusions | 40 |
| pAIN620 | pUSH1 expressing His6-McpA cytoplasmic domain | 24 |
| pMH1 | pMUTIN-HIS::mcpA | This work |
| pMH2 | pMUTIN-HIS::mcpB | This work |
| pMH3 | pMUTIN-HIS::mcpC | This work |
| pMH4 | pMUTIN-HIS::tlpA | This work |
| pMH4 | pMUTIN-HIS::tlpB | This work |
| pMH5 | pMUTIN-HIS::tlpC | This work |
| pMH6 | pMUTIN-HIS::yfmS | This work |
| pMH7 | pMUTIN-HIS::yoaH | This work |
| pMH8 | pMUTIN-HIS::yvaQ | This work |
| pTM18 | pGEX-6P-2::cheC2 | 42 |
| pTM25 | pGEX-6P-2::cheD2 | 42 |
| pVC13 | pGEX-6P-2:: hemAT | This work |
| pVC15 | pGEX-6P-2:: cheV | This work |
| pVC20 | pET-42b(+)::cheR | This work |
| pVC21 | pET-42b(+)::yfmS | This work |
| pVC43 | pMUTIN-YFP::yfmS | This work |
| pVC51 | pMUTIN-YFP::hemAT | This work |