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Western blot analysis of motility and chemotaxis proteins among mutants. Western blot analysis using B. burgdorferi monoclonal anti-FlaB, -FlaA, and -DnaK and E. coli polyclonal anti-CheA antibodies. Cell lysates (10 μg protein) from wild-type and cheY3 and cheY1 cheY2 cheY3 mutant (cheY1Y2Y3) cells were probed with the indicated antibodies. For FlaB, 3 μg was loaded in each lane, and for Dnak, 2 μg of lysate was loaded in each lane.
