Table 1.
Bacterial strains, plasmids, and phages used in this study
| Name | Genotype or description | Reference |
|---|---|---|
| Strain | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 (Strr) relA1 flbB5301 deoC1 ptsF25 rbsR | 4 |
| 2K1056 | F− λ−IN(rrnD-rrnE)1rph-1Δ (argF-lac)U169 | 26 |
| TR530 | MC4100 λRS88[spy-lacZ] | 29 |
| TR776 | MC4100 baeS1::Tn10cam | 27 |
| fimA::Kn | 2K1056 ΔfimA::Kn | This study |
| MC4100-amp | MC4100(pUC19) | This study |
| ΔbaeR-amp | TR1162(pUC19) | This study |
| CO4 | MC4100 mdtA::λplacMu53 | This study |
| SL99 | 2K1056 ΔbaeR::Kn | This study |
| SL100 | TR530 ΔbaeR::Kn | This study |
| SL102 | TR530 ΔmdtA::Kn | This study |
| SL103 | TR530 Δmtr::Kn | This study |
| SL113 | TR530 ΔtnaA::Kn | This study |
| SL114 | TR530 ΔsdiA::Kn | This study |
| SL119 | 2K1056 baeS1::Tn10cam | This study |
| SL131 | TR530 ΔacrF::Kn | This study |
| SL138 | MC4100 λRS88[mdtA-lacZ] | This study |
| SL142 | SL102 (pCA24N) | This study |
| SL143 | SL102 (pCA-mdtA) | This study |
| SL150 | SL138 ΔmdtA::Kn | This study |
| TR1162 | TR530 ΔbaeR | This study |
| Plasmid | ||
| pUC19 | Ampr cloning vector; used for β-lactamase production in the study | Invitrogen |
| pRS415 | bla-T14-EcoRI-SmaI-BamHI-lacZ+ | 34 |
| pCA24N | Vector control for the ASKA library | 11 |
| pCA-mdtA | pCA24N containing the mdtA gene behind the pT5-lac promoter (ASKA library) | 11 |
| Phage | ||
| λRS88 | bla′-lacZsc | 34 |