Chitinases produced in vitro by F. tularensis (A1a, A1b, A2, and type B) and F. novicida. Western blots of WCL (15 μg) of each Francisella strain with anti-ChiA antiserum, anti-ChiB antiserum, and anti-ChiC antiserum. Lanes 1 to 3, A1a strains OK01-2528, MO02-4195, and SCHU S4, respectively; lanes 4 and 5, A1b strains MA00-2987 and MD00-2970, respectively; lanes 6 and 7, A2 strains NM99-1823 and WY96-3418, respectively; lanes 8 to 10, type B strains KY99-3387, LVS, and MI00-1730, respectively; lanes 11 and 12, F. novicida strains GA99-3548 and GA99-3550, respectively.