Fig. 1.

B. hermsii pFAEV3 suicide vector map and PCR-based verification of allelic exchange. A suicide vector (pFAEV3) was constructed to allow for the introduction of a selectable marker (kan) and a green fluorescent protein (gfp) gene into a noncoding region of the native B. hermsii linear plasmid lp200. (A) The vector map is depicted. The primers used to generate the amplicons for vector construction (VC) are indicated. (B) Schematic depicting lp200 after cassette integration. The location of the primer targets for PCR used to confirm integration (IC primers) are indicated. The ethidium bromide-stained amplicons obtained through the PCR analyses are also shown. Primer sequences can be found in Table 1. Bh, B. hermsii; wt, wild type.