Table 1.
Oligonucleotides used in this studya
| Primer | Sequence (5′ to 3′) |
|---|---|
| VC1 | GTCAATGGTATATTAGTTTCAAGTTCTATGACCC |
| VC2 | ACGCGTAAAGTCGACTTAACATATAAATAATACTGATATTAAAATTTATGTAAA |
| VC3 | GTCGACGTTAAAGAAAATTGAAATAAACTTGGACTATGTTAATG |
| VC4 | GGCGCGCCTTAGAAAAACTCATCGAGCATCAAATG |
| VC5 | GGCGCGCCGGGCGAATTCGGCTTATTCC |
| VC6 | GACGTCCTATTTGTATAGTTCATCCATGCCATGTG |
| VC7 | GACGTCCCTGAGAGTATAGGCAGTACTTCAATTACTACC |
| VC8 | ACGCGTCAATTGATATTGATGTGGCGTTGATTTCTAAAATAG |
| IC1 | GTCTACTCTATCTGGGATACTAAAGAGC |
| IC2 | CAAATTTCAATGACTTGCAAAATTTAGAGC |
| IC3 | CATCATTGGCAACGCTACC |
| IC4 | GTCCACACAATCTGCCCTTTC |
| IC5 | ATTGCATTAAGTTATGCAGATAAATTTAAGC |
| IC6 | GCATTAAGTAAGCGGTTTGTGAGC |
a
Abbreviations used in primer designations are as follows: VC, vector construction, and IC, insert confirmation. Restriction site tails are indicated by underlining.