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Cell wall protein localization is defective in a cwp84 mutant. C. difficile 630Δerm (WT) and the cwp84 mutant harboring plasmids (see below) were grown in BHI broth to late exponential phase (OD600 = 0.5). Cell wall extracts and culture supernatants were prepared and analyzed by SDS-PAGE, followed by Western blotting with antibodies against Cwp66 and Cwp2. Plasmids: –, pMTL960; 84, pCwp84WT; 84*, pCwp84C116A.
