Fig. 4.

Expression and secretion of CopN in the presence or absence of Scc1, Scc4, and Scc3. Y. pestis KIM8 Δ4 (parent) (A) and KIM8-P109 ΔyopN-sycN-yscB-tyeA) (B) alone or carrying plasmids pCopN, pCopN/Scc1, CopN/Scc4, and pCopN/Scc1/Scc4 or plasmids pCopN/Scc1/Scc4 and pScc3 were cultured in TMH medium with (+) and without (−) 2.5 mM calcium for 5 h at 37°C. Secreted (S) and/or cell pellet (P) proteins were analyzed by SDS-PAGE and immunoblotting with antisera specific for CopN, Scc1, Scc4, Scc3, YopM, or H-NS.