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Expression and secretion of CopN in the presence or absence of Scc3. Y. pestis KIM8 Δ4 carrying plasmid pCopN or pCopN/Scc1/Scc4 with and without plasmid pScc3 was cultured in TMH medium with (+) and without (−) 2.5 mM calcium for 5 h at 37°C. Secreted (S) and/or cell pellet (P) proteins were analyzed by SDS-PAGE and immunoblotting with antisera specific for CopN, Scc1, Scc4, and Scc3.
