Table 1.
Strains and plasmids used in these studies
| Strain or plasmid | Characteristics | Reference |
|---|---|---|
| Strains | ||
| D39 | Wild-type, type 2 encapsulated | 1 |
| D39-Smr | D39, streptomycin resistant | This study |
| D39-C0832:1 | D39 dldh::pSH0832, DLDH negative | 41 |
| D39-P2A1 | D39 dldh-lplA intergenic::pSH08P | 41 |
| CP1296 | Rx derivative, cbp3::kan-rpsL+ | 44 |
| D39-ΔRafK | D39,ΔrafK::kan-rpsL+, RafK negative | This study |
| D39-rRafK | D39, allelic repair rafK, RafK expressing | This study |
| D39-ΔRafK-Janus | D39 ΔrafK::kan-rpsL+, RafK negative | This study |
| D39-rRafKΔlipErm | D39 allelic repair rafKΔlip, Ermr | This study |
| D39-ΔRafE | D39 rafE::pRT-ΔrafE, RafE-negative | This study |
| Plasmids | ||
| pJY4164 | Ermr, ori E. coli | 54 |
| pQE30 | Expression vector, Ampr | Qiagen |
| pAH001 | pQE30::dldh (full length) | 17 |
| pRT-JanusBglII | pGEM-T Easy::kan-rpsL+ with BglII ends | This study |
| pRT-ΔrafK | pGEM-T Easy::rafK deletion overlap PCR | This study |
| pRT-ΔrafK-Janus | pGEM-T Easy::rafK deletion with kan-rpsL+, inserted | This study |
| pRT-rRafKΔlipErm | pGEM-T Easy::rafKΔlip (amino acids 1 to 251 + Erm cassette amplified from pJY4164) | This study |
| pRT-ΔrafE | pJY4164::rafE internal fragment | This study |
| pRT-RafK | pQE30::rafK (full length) | This study |
| pRT-RafKΔlip | pQE30::rafKΔlip (amino acids 1 to 251) | This study |
| pRT-RafKLipo | pQE30::rafK lipoyl domain only (amino acids 246 to 377) | This study |