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Nitrate and nitrite assimilation in wild-type Anabaena sp. PCC 7120 and the Δhfq mutant strain. To determine nitrate (A) and nitrite (B) assimilation in Anabaena wild-type or Δhfq cells, 100 μM sodium nitrate or sodium nitrite was added to the medium and incorporation was measured over a time course. Nitrite assimilation was measured at pH 7.5 and pH 9.6. Assimilation was determined in three independent experiments, with similar results, and a representative example is shown.
