Table 1.

Primers used for PCR amplification and/or sequencing of the B. anthracis four-gene anthrose operon (BAS3319 to BAS3322)

Target gene	Primer	Purpose	Sequence (5′→3′)
BAS3319	BAS3319_F	Amplification and sequencing	TGGAATCGCAACGATGAATA
	BAS3319_R	Amplification and sequencing	AAAACGCTAGAAAGAACCTCTG
	BAS3319_S9	Sequencing	CCCCATTAATGCATTTCCAC
	BAS3319_S10	Sequencing	AGGGGAAGTTGGGATTGAAA
BAS3320	BAS3320_F1	Amplification and sequencing	AGCGGTAGTGGTTCCGTATG
	BAS3320_R1	Amplification and sequencing	CCCCATTAATGCATTTCCAC
	BAS3320_F2	Amplification and sequencing	TGGGTGGGGAATTATTCAAG
	BAS3320_R2	Amplification and sequencing	TATTCATCGTTGCGATTCCA
	BAS3320_S7	Sequencing	CGAAATCGTATCTCCGAATG
	BAS3320_S8	Sequencing	GCATGAGCGCATATTTTCCT
BAS3321	BAS3321_F1	Amplification and sequencing	GAACCGACAACGGTTTTGTT
	BAS3321_R1	Amplification and sequencing	GCATGAGCGCATATTTTCCT
	BAS3321_F2	Amplification and sequencing	TTCGCATGGCATTATAGCTG
	BAS3321_R2	Amplification and sequencing	GGAAAATTCCCCAAATCCTT
	BAS3321_F3	Amplification and sequencing	GAGTACCGCGTTTCACCAAT
	BAS3321_R3	Amplification and sequencing	ATGGCGTTTCATTTTTCACA
	BAS3321_S1	Sequencing	CTCGCATTGCTTTTTCATCA
	BAS3321_S2	Sequencing	TGCGTACATTGAAACGGAAA
	BAS3321_S3	Sequencing	GGTTGCCTTCCCCATCTATT
	BAS3321_S4	Sequencing	AGGCAACCTATTGCCAGATG
BAS3322	BAS3322_F2	Amplification and sequencing	TTTCAAAATAATGCGGACGA
	BAS3322_R	Amplification and sequencing	TGATGGATTTCTAAAAAGGGAAA
	BAS3322_S5	Sequencing	CAACCTCCGCCTAGTGCTAA
	BAS3322_S6	Sequencing	TCTCGAGGAGAGGCGTTAAA