Table 2.
Sequence polymorphisms in the BAS3320 gene (1,139 bp, encoding aminotransferase) of the anthrose operon
| B. anthracis straina | Polymorphism (amino acid) |
|
|---|---|---|
| T→G | Tandem repeat | |
| Sterne | 70 ATT 72 (I) | 211 TTTAAAAAAAGAAAAAAAG--------GAA 232 |
| A1055 | 70 ATG 72 (M) | 211 TTTAAAAAAAGAAAAAAAG--------GAA 232 |
| KrugerB | 70 ATT 72 (I) | 211 TTTAAAAAAAGAAAAAAAG--------GAA 232 |
| A0442 | 70 ATT 72 (I) | 211 TTTAAAAAAAGAAAAAAAG--------GAA 232 |
| A0465 | 70 ATT 72 (I) | 211 TTTAAAAAAAGAAAAAAAG--------GAA 232 |
| CNEVA-9066 | 70 ATT 72 (I) | 211 TTTAAAAAAAGAAAAAAAG--------GAA 232 |
| Zimbabwe | 70 ATT 72 (I) | 211 TTTAAAAAAAGAAAAAAAG--------GAA 232 |
| Mali | 70 ATT 72 (I) | 211 TTTAAAAAAAGAAAAAAAGAAAAAAAGGAA 240 (frameshift → stop) |
| Chad (13x) | 70 ATT 72 (I) | 211 TTTAAAAAAAGAAAAAAAGAAAAAAAGGAA 240 (frameshift → stop) |
| Cameroon (6x) | 70 ATT 72 (I) | 211 TTTAAAAAAAGAAAAAAAGAAAAAAAGGAA 240 (frameshift → stop) |
a
The B. anthracis strains from Zimbabwe, Mali, Chad, and Cameroon were isolated from cattle carcasses. The B. anthracis strains Sterne, A1055, KrugerB, A0442, A0465, and CNEVA-9066 are representative hits from sequence comparisons using the BLAST program (http://blast.ncbi.nlm.nih.gov/Blast.cgi).