Table 2.
Type 3 fimbrial production of Klebsiella strains
Culture condition | Serum titera |
||||
IApc35 | IApc35 mrkI::Knr | IApc35 mrkI::Knr plus mrkI | IApc35 ΔmrkHI | IApc35 ΔmrkHI plus mrkHI | |
---|---|---|---|---|---|
Aerobic (shaken flask) | 5,120 | <40 | 40,960 | <40 | 40,960 |
Aerobic (agar grown) | 5,120 | <40 | 40,960 | <40 | 40,960 |
Microaerophilic (static tube) | 40,960 | 20,480 | ND | <40 | 40,960 |
Anaerobic (agar grown) | 5,120 | 10,240 | ND | <40 | 40,960 |
The serum titer represents the reciprocal of the anti-MrkA serum dilution needed to produce visible agglutination. The lowest dilution of serum used was 1:40. ND, not determined.