Fig. 4.

Mass spectrometry analyses of tryptic peptides from flagellin proteins of B. thailandensis E264. nLC-MS/MS spectrum of the triply protonated T^185–213 glycopeptide ion of m/z 1074.2 from B. thailandensis. The spectrum is dominated in the low-molecular-m/z region by the carbohydrate oxonium ion (m/z 343.1) and what could be fragmentation components (m/z 301.1 and 283.1). From the observed mass of the glycopeptide, a mass excess of 342 Da was observed, corresponding to a single glycan modification. Below the graph is the amino acid sequence of the B. thailandensis E264 FliC protein. The sequence of the T^185–211 glycopeptide is underlined. Sequences in bold indicate unmodified peptides observed in tryptic digests.