Fig. 5.

Electrospray mass spectrometry of B. pseudomallei K96243 and B. thailandensis E27 glycans. Second-generation product ion spectra of ions obtained from in-source dissociation of multiply charged flagellin ions. MS/MS spectra were acquired by increasing the radio frequency (RF) lens 1 voltage from 40 to 90 V, forming fragment ions in the orifice/skimmer region of the mass spectrometer and promoting the formation of labile ions from the intact flagellin MS/MS spectrum of B. pseudomallei K96243 m/z 292.1 (a) and B. thailandensis E264 m/z 343.1 (b). This yielded a series of predominant fragment ions which did not correspond to peptide type y and b ions. Plausible neutral losses of molecules such as acetyl groups and water are indicated.