Table 5.
Quantitative analysis of flow cell biofilms formed by WT P. aeruginosa PA14 with different additives by day 6a
| Additive(s) to biofilm control medium (1% TSB) | No. of images | Total biomass (μm3/μm2) | Substratum coverage (%) | Avg thickness of biomass (μm)b | Maximum biofilm thickness (μm) |
|---|---|---|---|---|---|
| None | 1 | 7.20 | 46.7 | 16.4 | 62 |
| 40 μg/ml conalbumin | 6 | 0.80 ± 0.08 | 11.8 ± 1.3 | 9.0 ± 0.7 | 32 |
| 40 μg/ml conalbumin, 10 μM PCA | 4 | 10.70 ± 0.49 | 80.0 ± 4.3 | 13.2 ± 0.8 | 36 |
| 40 μg/ml conalbumin, 10 μM PYO | 1 | 0.34 | 4.2 | 8.7 | 32 |
a
Biofilms were formed at 22°C for 6 days with no addition or with the addition of 40 μg/ml conalbumin alone or together with 10 μM phenazine (PCA or PYO) to 1% TSB medium. Representative day 6 images from this data set are shown in Fig. 4B. All values are means of the results for n images ± the standard error of the mean. Each image covers an area of 3.03 × 105 μm2.
b
Calculated for the area covered by the biomass.