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. 2011 May;31(10):2111–2121. doi: 10.1128/MCB.01159-10

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Copyright © 2011, American Society for Microbiology

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Fig. 3. — Substantial association between Ap₄A hydrolase and importin beta in RBL cells. (A) The protein sequence of Ap₄A hydrolase was subject to domain-domain association prediction analysis using bioinformatic tools. The Nudix domain was predicted to associate with the importin beta N-terminal domain (IBN_N) by the putative interaction database InterDom (http://interdom.i2r.a-star.edu.sg/). (B) Coimmunoprecipitation analysis demonstrating the time course of Ap₄A hydrolase-importin beta interaction following IgE-Ag stimulation, using anti-Ap₄A hydrolase antibody for immunoprecipitation (IP) and either anti-importin beta or anti-Ap₄A hydrolase for immunoblotting. The specific Ap₄A hydrolase band was verified by a control experiment using siRNA to knock down Ap₄A hydrolase (right lanes). Data for one representative experiment of six are shown.