Skip to main content
. 2011 May;31(10):2090–2099. doi: 10.1128/MCB.01137-10

Fig. 1.

Fig. 1.

Human RECQ5 prevents DNA breaks during S phase. (A) Human 293T cells were transfected with control or RECQ5 siRNA for 24 or 48 h. Expression levels of RECQ5 (top) or actin (center) were determined by Western blot analysis of whole-cell extracts. Chromosomal DNA integrity was analyzed by standard agarose gel electrophoresis (bottom). (B) Representation of RECQ5 knockdown cells with γH2AX foci (red) and CIdU replication foci (green). (C) Quantification of the percentage of CIdU-positive cells containing γH2AX foci transfected with either control or RECQ5 siRNA. (D) Flow cytometry of the control (top panels) and RECQ5 knockdown cells (bottom panels) 3, 10, or 14 h after release from nocodazole (Noc) treatment. (E) Representative images of the neutral comet assays using control and RECQ5 knockdown cells harvested at the 3rd, 10th, or 14th hour after Noc treatment. (F) Average tail moment measured from >200 cells per sample from panel E.