Fig. 6.

Neph1 localization at the podocyte cell membrane is altered in the presence of dominant negative Myo1c. (A and C) Human podocytes transfected with wt GFP-Myo1c or dominant mutant GFP-Myo1c (without ATP and actin binding domains) were analyzed by immunofluorescence with Neph1 (A) or ZO-1 (C) antibodies. Note the presence of Neph1 and ZO-1 at the cell membrane (arrows) in untransfected cells and cells transfected with wt Myo1c. In contrast, cells transfected with mutant GFP-Myo1c showed a significant reduction in the amount of Neph1 but not of ZO-1 at the cell membrane. (B) Quantitative analysis of transfected and untransfected cells was performed (n = 10 cells), and the bar diagram shows the mean pixel intensities of Neph1 at the cell periphery.