Fig. 9.

Clustering of Neph1 at the podocyte membrane is inhibited in Myo1c-deleted cells. (A) A plasmid encoding the CD16-Neph1 cytoplasmic domain was transfected into Myo1c knockdown and control cells. (Right) Live cells were processed for clustering with CD16 antibody and analyzed by immunofluorescence. (Left) A parallel set of untransfected cells (control) was immunostained with Neph1 and Myo1c antibodies to determine the extent of the Myo1c knockdown. Representative images from three independent experiments are shown. (C) The same cells were also analyzed by confocal microscopy. (B) Quantitative analysis of transfected cells (n = 10 cells) shows a significant decrease in the mean pixel intensity of CD16-Neph1 in Myo1c knockdown cells compared to control cells.