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. 2011 May;31(10):1972–1982. doi: 10.1128/MCB.00981-10

Fig. 1.

Fig. 1.

BMI1 and CBX2 are recruited to sites of DNA damage. (A) HeLa cells were subjected to laser scissors-induced DNA damage and, after 30 min, localization of pH2AX and either BMI1 (top panel) or CBX2 (bottom panel) was assayed by IF. The percentage of cells with colocalization of pH2AX with BMI1 or CBX2 is indicated (right panel). (B) The top panels show HeLa cells treated with either 0 Gy of IR (−IR) or 10 Gy of IR (+IR). After 8 h, the localization of BMI1 and pH2AX was assayed by IF. In the bottom panels, HeLa cells were treated with either no or 3 mM HU for 2 h, and localization of BMI1 and 53BP1 was assayed by IF. In the right panel, the mean numbers of BMI1 foci colocalizing with pH2AX foci per cell are plotted for each condition. The data shown are means ± the standard error of the mean (SEM) from at least three separate experiments.