Skip to main content
. 2011 May;31(10):1972–1982. doi: 10.1128/MCB.00981-10

Fig. 4.

Fig. 4.

(A) Bmi1+/+; Ink4a−/−; Arf−/− MEFs, Bmi1−/−; Ink4a−/− Arf−/− MEFs, H2AX−/− MEFs, or H2AX−/− MEFs expressing S139A mutant H2AX were treated with laser scissors and, after 30 min, processed for IF using antibody to BMI1 and either 53BP1 or pH2AX as indicated. A bar graph shows the percentages of cells with laser scissors-induced damage showing colocalization of BMI1 with 53BP1 in each MEF strain. The data plotted are the mean ± the SEM of three separate experiments. (B) In the top two panels, HeLa cells were treated with either 0 or 0.5 μM ATMi and subjected to laser scissors and, after 30 min, processed for IF using antibodies to BMI1 or 53BP1. In the middle two panels, Seckel cells or Rnf8−/− MEFs were treated with laser scissors and processed for IF using the antibodies indicated. In the bottom two panels, HeLa cells were treated with either control siRNA or ATR-specific siRNA and then treated with lasers scissors and processed for IF using antibodies to BMI1 and 53BP1. Western blots showing the effect of siATR on ATR protein levels are shown below the panels. Bar graph shows quantitation of percentage of cells that had clear colocalization of BMI1 with 53BP1 or pH2AX. The data are plotted as means ± the SEM of at least three separate experiments.