Fig. 2.

Autophagy plays a role in lysosomal ferritin degradation in iron-deficient MEFs. (A) Suppression of Dfo-induced ferritin degradation by 3-MA. MEFs pretreated with FAC were cultured in Dfo in the presence or absence of 3-MA for the indicated times. (B to D) The reduction in ferritin in response to iron chelators is suppressed in autophagy-deficient cells. WT or Atg5 KO MEF cells pretreated with FAC were cultured in the presence of Dfo (B), Dfx (C), or BPS (D) for the indicated times. For the experiments whose results are shown in panels A to D, cell lysates were probed as described in the legend to Fig. 1A. (E, F) Attenuation of Dfo-induced ferritin degradation in autophagy-deficient MEFs. WT and Atg5 KO (E) or Atg7 KO (F) MEFs were pulse-labeled as described in the legend to Fig. 1B. The amounts of ferritin L chain (upper bands) in WT and Atg5 KO (E) or Atg7 KO (F) MEFs were quantified. Error bars show standard deviations. *, P < 0.05.