Table 1.
Results of a representative LC-MS/MS analysis of AXIN1 and AXIN2 affinity-purified protein complexesa
| Protein | No. of peptides |
Protein accession no. | No. of pulldowns | |
|---|---|---|---|---|
| Total | Unique | |||
| AXIN1-type proteins | ||||
| AXIN1 | 197 | 47 | NP_003493.1 | 4 |
| Ubiquitin C | 46 | 42 | NP_055524.2 | 1 |
| GSK3α | 36 | 23 | NP_002084.2 | 4 |
| USP34 | 35 | 28 | NP_055524.3 | 3 |
| GSK3β | 33 | 22 | NP_063937.2 | 4 |
| Ubiquitin B precursor; polyubiquitin B | 18 | 1 | NP_066289.1 | 1 |
| PP2A regulatory subunit B56ε | 11 | 7 | NP_006237.1 | 3 |
| WD repeat domain 26 | 11 | 9 | NP_079436.3 | 3 |
| PP2 regulatory subunit Aα | 9 | 6 | NP_055040.2 | 3 |
| β-Catenin | 7 | 7 | NP_001895.1 | 1 |
| Armadillo repeat containing 8 | 6 | 3 | NP_056211.2 | 2 |
| PP2 catalytic subunit α | 5 | 3 | NP_002706.1 | 3 |
| PP2 catalytic subunit β | 5 | 3 | NP_004147.1 | 3 |
| RAN binding protein 9 | 5 | 4 | NP_005484.2 | 3 |
| Yippee-like 5 | 5 | 4 | NP_057145.1 | 3 |
| 8-Oxoguanine DNA glycosylase isoform 2d | 3 | 2 | NP_002533.1 | 1 |
| APG4 autophagy 4 homolog B | 3 | 2 | XP_001126088.1 | 1 |
| RAN binding protein 10 | 3 | 3 | NP_065901.1 | 3 |
| APC | 2 | 2 | NP_000029.2 | 4 |
| CK1α | 2 | 2 | NP_660204.1 | 4 |
| DEP domain containing 2 isoform a | 2 | 2 | NP_079146.2 | 1 |
| PP2A regulatory subunit B56-γ | 2 | 2 | NP_848701.1 | 3 |
| AXIN2-type proteins | ||||
| AXIN2 | 155 | 19 | NP_004646.2 | 4 |
| Nucleolin | 70 | 10 | NP_005372.2 | 1 |
| GSK3β | 42 | 14 | NP_002084.2 | 4 |
| GSK3α | 34 | 12 | NP_063937.2 | 4 |
| β-Catenin | 28 | 9 | NP_001895.1 | 2 |
| APC | 27 | 16 | NP_000029.2 | 4 |
| USP34 | 21 | 13 | NP_055524.3 | 3 |
| Junction plakoglobin | 9 | 6 | NP_068831.1 | 3 |
| PP2 catalytic subunit α/β | 5 | 4 | NP_002706.1 | 2 |
| abl-interactor 1 | 5 | 3 | NP_005461.2 | 1 |
| PP2 regulatory subunit Aα | 3 | 3 | NP_055040.2 | 1 |
| Wiskott-Aldrich syndrome protein family member 2 | 3 | 2 | NP_008921.1 | 1 |
| PP2A regulatory subunit B56γ | 2 | 2 | NP_848701.1 | 1 |
| α-Catenin | 2 | 1 | NP_001894.2 | 1 |
| Nucleoporin | 2 | 2 | NP_055484.2 | 1 |
| Wiskott-Aldrich syndrome protein family member 1 | 2 | 2 | NP_003922.1 | 1 |
a
The total peptides column indicates the total number of peptides successfully matched to protein identity, whereas the unique peptides column gives the number of distinct peptide fragments identified. Four independent purifications of AXIN1 and AXIN2 complexes were analyzed by LC-MS/MS, and the results of a representative pulldown experiment are shown in this table. The frequency with which a protein was identified in the four pulldowns is noted in the “No. of pulldowns” column.