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. 2011 Jul;31(13):2641–2652. doi: 10.1128/MCB.01341-10

Fig. 5.

Fig. 5.

Effect of siRNA-mediated knockdown of Brd4 ET interactors on Brd4-dependent E2-mediated transcriptional activation of the BPV1 LCR. C33A/E2TA/BPV1LCRc2 cells were transfected with the indicated siRNA duplexes. (A) Knockdown of NSD3, JMJD6, CHD4, and CDK9 protein levels shown by resolving equal amounts of lysates by SDS-PAGE and immunoblotting with the indicated antibodies or the control, actin. (B) Effect of knocking down Brd4 ET interactors on BPV1 LCR reporter activity was quantified by measuring luciferase activity 72 h posttransfection and normalizing the relative luciferase units (RLU) to total protein concentrations from three replicate experiments. Equal amounts of lysates were resolved by SDS-PAGE and immunoblotted with the indicated antibodies to determine knockdown levels of the proteins targeted by siRNA or the control, actin.