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. 2011 Jul;31(13):2707–2714. doi: 10.1128/MCB.05133-11

Fig. 1.

Fig. 1.

CPEB regulation of IL-6 mRNA translation. (a) Sequence comparison of a portion of the IL-6 3′ UTR in humans, dogs, and mice. The putative CPEs are highlighted in gray, and the polyadenylation hexanucleotide is in lowercase. (b) ELISA determination of levels of IL-6 secreted into the medium of cultured WT and CPEB KO immortalized MEFs. (c) IL-6 levels secreted by CPEB KO MEFs transduced with virus expressing FLAG-CPEB or FLAG only. The Western blot shows FLAG-CPEB expression. (d) CPEB KO MEFs were cotransfected with FLAG-CPEB or the empty vector together with a Renilla luciferase reporter plasmid containing the 3′ UTR of mouse IL-6 and a firefly luciferase (Luc) vector as a normalization control. The black bar refers to the ratio of Renilla to firefly luciferase expression as a percentage of the control, and the gray bar indicates the ratio of Renilla to firefly luciferase RNA as measured by quasiquantitative RT-PCR. (e) CPEB KO MEFs transduced with FLAG-CPEB- or FLAG-expressing virus were used for RNP coimmunoprecipitation with FLAG-tagged antibody. RNA was extracted from the precipitate and examined for IL-6 and KC RNAs by RT-PCR. Asterisks indicate P values of <0.001.