Fig. 3.

Cytokine production in WT and CPEB KO MEFs. (a) Cytokine levels produced by immortalized CPEB KO MEFs. A mouse cytokine antibody array was used to measure the levels of the indicated cytokines produced by WT and CPEB KO MEFs at the early (presenescent), senescent, and late stages. The values indicate the ratios of CPEB KO to WT levels. (b) Determination of relative IL-6 RNA levels from early (P2)- or late (P20)-passage immortalized MEFs by RT-PCR. GAPDH served as a control. (c) RNA from WT or CPEB KO immortalized MEFs was used for quasiquantitative RT-PCR analysis using intron- or exon-specific primers for IL-6 and KC RNAs. GAPDH served as a control. The values above the panels indicate the degrees of dilution of the starting RNA.