Fig. 2.

2D gel electrophoresis of human U4/U6.U5 tri-snRNP proteins. Tri-snRNPs were purified as described in Materials and Methods, and their associated proteins were separated by 2D gel electrophoresis under conditions used for high-molecular-mass proteins and analyzed as described for Fig. 1. (A) Sypro Ruby staining. (B) Pro-Q Diamond staining. Note that the tri-snRNPs analyzed here are not highly pure, and thus multiple minor spots, which represent contaminants, are also visible.