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. 2011 Jul;31(13):2653–2666. doi: 10.1128/MCB.01358-10

Fig. 4.

Fig. 4.

The association between ADAP and WASp is independent of SLP76. E6.1 Jurkat T cells or SLP76-deficient J14 cells stably reconstituted with WASp-CFP were transfected with ADAP-YFP. Cells were plated, and FRET efficiency between ADAP and WASp was determined. No significant difference was measured between the two molecules in the presence versus the absence of SLP76 (P ≤ 0.06). 1.0′, 3.0′, and 5.0′ indicate minutes of activation. (B) FRET analysis of the molecular associations between WASp and ADAP. J14 or E6.1 cells expressing WASp-CFP and ADAP-YFP were seeded and were fixed 3 min after the initiation of the spreading process. The average value of FRET efficiency ± standard error obtained for a given pair is shown. The data shown are representative of >3 independent experiments.