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. 2011 Jul;31(14):2867–2876. doi: 10.1128/MCB.05430-11

Fig. 4.

Fig. 4.

Autophagy induction potential of RAD001 correlates with mTOR complex 1 stability. (A) U2OS, H4, RT112, SK-N-SH, and HN10 mCherry-GFP-LC3 cell lines were lysed in RIPA buffer and analyzed by Western blotting using the indicated primary antibodies. (B) U2OS, H4, RT112, SK-N-SH, and HN10 mCherry-GFP-LC3 cell lines were treated for 18 h with 0.1% DMSO or 250 nM RAD001. Half of the dishes were subjected to chemical cross-linking using 1 mg/ml DSP. Cell lysates were prepared using 0.3% CHAPS, immunoprecipitated using anti-mTOR antibody, and analyzed by Western blot analysis.