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. 2011 Jul;31(14):2817–2826. doi: 10.1128/MCB.01305-10

Fig. 1.

Fig. 1.

(A) RUNX1 binds the SMAD6 promoter and conserved noncoding regions in K562 cells. The upper panel shows a VISTA plot of human/mouse sequence conservation greater than 50% across the SMAD6 locus. Noncoding sequences with greater than 75% conservation are shaded in pink, exons in magenta, and the untranslated regions in cyan. The middle and lower panels show ChIP-seq traces using a nonimmune serum (NI serum) control and a Runx1 antibody in K562 human erythroleukemic cells. Enrichments at the human SMAD6 (HsSMAD6) promoter and a region 71 kb upstream of the promoter are marked with arrows. (B) Runx1 binds a region at kb −57 in mouse HPC-7 stem/progenitor cells. In contrast with K562 cells, Runx1 enrichment in mouse blood stem cells is seen mostly at a region 57 kb upstream of the mSmad6 promoter, which is homologous with the human region at kb −71. (C) There are active chromatin marks and Runx1 enrichment by ChIP-RT-PCR at sites identified by ChIP-seq. (i) Human K562 erythroleukemic cells; (ii) mouse HPC-7 stem/progenitor cells.