Fig. 2.

SCCA1 enhances ER stress-induced cell death. (A and B) Parental bax^−/− bak^−/− BMK cells or those expressing Flag-SCCA1 or Flag-SerpinB3b were treated with tunicamycin (A) or thapsigargin (B). Cell death was measured by PI exclusion. (C and D) Cells were left untreated or treated with tunicamycin for 16 h. Cells were stained with LysoTracker Red and DAPI. Cells were photographed (C) or subjected to flow cytometry (D). (E) Cells were treated with tunicamycin for the indicated times. The ER stress response was examined by immunoblotting with indicated antibodies. (F) MCF10A parental cells, SCCA1-expressing cells, and SCCA1-expressing cells containing a Tet-inducible shSCCA1 either left untreated or treated with doxycycline (Dox) for 3 days were treated with either tunicamycin or thapsigargin. Cell death was measured by PI exclusion.